FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859051
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATT17102.0008883E-119.4123171
CTCACAT13051.6057129E-78.9697321
CTTAGAG8700.0087016337.56821161
CCTCAAT24505.620677E-107.16663271
CTCAGAT14606.15481E-57.0152821
CTTCATT43150.06.9514121
CTCTAAA14757.039212E-56.9439411
CCCAGAG12656.563177E-46.94001961
CTAAGAT10550.00617274366.9345381
CCCACTT16002.7555243E-56.85869171
CCCAAAT18253.3911838E-66.8148461
CCCACGT10850.0080960566.74279981
CCTGGGA10850.0091632366.6568078
CTCTTAT24252.4843757E-86.6371391
CCCAAAG20002.0085117E-66.58434341
CTCATTT30552.3101165E-106.4658041
CTCGACT12650.00498773956.36168431
CCCAAAA27758.272764E-96.3272971
CCCGAAT15107.025035E-46.2984891
CTTAAAT25758.127972E-86.25050971
Produced by FastQC (version 0.11.5)