FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879258
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTT4600.00641689710.9519722
ATTCGTG4600.006422329710.9507266
CCCTTTT10457.1211307E-69.1181931
AGTTATT6400.0070709028.9952394
CTCTCTT9903.7402897E-58.8843931
TTGTGTA6600.0091285598.7191849
CTCAAAG11251.6632442E-47.81826541
AGACAAT9900.00325864067.4036611
TTCAATT12104.7510923E-47.13753032
CTTCCAT14404.992525E-57.12602331
CTTCAAT13501.7470085E-47.05815651
CTCTTTT21058.6456566E-86.9640131
CAACAAA12450.0041339356.4759731
CATTGAT14905.828453E-46.39497381
ATTTTGT15450.0074388895.5892756
ACGTCGC3300.0083411435.5327353140-144
Produced by FastQC (version 0.11.5)