FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930896
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT24456.87578E-107.10034471
CTTAAAC15902.9575229E-56.82403371
CCCCAAC14000.00235083866.2001221
CCCAAAA29706.9776434E-96.08878471
CTCAGCG18051.705256E-46.0111991
AAGGGTG19708.965549E-55.87009339
CTCAAGA22651.82183E-55.74845741
CCTCTAT18953.3077874E-45.7257071
CCCACAA17758.9319266E-45.7052771
CTCCGAC21605.443598E-55.69301371
CCTCGAA21806.261314E-55.6407841
CTTGTGT15600.0078337055.56421231
CTTTGGT25051.5490972E-55.4864691
CTCGGAT15900.0096601425.4592271
CTCCAAA28203.5412559E-65.3866311
CCCGAAC18850.00191157945.3723431
CTTTTAT59400.05.35813051
CCTCGAT26153.1698244E-55.25568061
CCCAAAT18000.0065096565.22417741
CTACGAA19500.00292462765.1932651
Produced by FastQC (version 0.11.5)