FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701039
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGT4202.0556498E-413.738881
CCCACTC3200.00745821313.524211
CCCGTCT4302.5092054E-413.4193711
CCCTCAT4300.003816485611.7419491
CCCAAGG4400.0045313311.4750861
CTTGGAT5400.00170087410.6857951
CTCAAAG8951.2079072E-610.4768541
CTCATAA6759.938683E-49.6172161
CTCGAAG8701.06529E-49.1197741
CGATTTT9452.7091373E-48.3678344
CTTTCAA12408.460986E-68.143611
CAATAAA10155.6354015E-47.8169491
CAACGAT8400.00720434127.728121
AAAAGGT9800.00356504417.33543873
CTTCTCT13903.8667626E-57.2648031
CTTCATT11350.00185026256.99048661
CTTCAAT11650.0024360876.81047441
CTTCAAA12808.794362E-46.7621051
CATCAAT15251.3051677E-46.6216891
CAACAAC12050.0034724156.58440071
Produced by FastQC (version 0.11.5)