FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608246
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC600.004716677614.49457125-129
GAAACCC3150.00668180813.7503347
TCCAAGC5150.001136765611.2138647
CGATAAA7850.00357238728.35477351
CACAAAG8950.00127836658.1421451
AAAGATT15301.7639048E-57.07795864
CTTCAAA10950.0091626946.6549951
CAAAAAG16550.00220375235.72411