FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences872904
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAAT14901.3674071E-78.3399591
CTCCAAA9400.0020135167.77630851
CTTCGAT11953.4447171E-47.3403141
CCTTGTT12807.5622636E-46.85287141
ACGGCAG10850.0092513996.64907179
CTTCTGT13850.00184686466.33333971
CCACCAT12800.00567803356.2817991
CTTTATT14600.00333619726.0079971
CTTTGAA13450.0094865755.97821761
GAAAGAG15300.00648441355.6592023
TCCTTTT19700.0034172915.12775473
CATCAAT18800.0095430865.05460071