FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836151
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3700.034.4451877
ACACGTC4100.031.0846828
CACGTCT4100.031.0846829
GAGCACA5850.022.996174
AGAGCAC6350.021.1829153
GCACACG7100.019.9435946
AGCACAC6500.019.6060895
AAGAGCA11000.012.8742242
GAAGAGC11653.4742698E-1010.9626221
AACCGGG7703.8173664E-49.1947746
AGTCACT3209.489668E-98.4071720-24
CCTGAAT9050.00185797187.8400591
ACTGACT4253.4197E-97.32950725-29
CCAGTCA4501.0761141E-86.92239520-24
TCTCGTA4151.2623605E-76.823746735-39
CTCGTAT4055.9805825E-76.642622535-39
ACTCCAG5204.154572E-96.53434615-19
CAGTCAC5001.2674718E-86.51334520-24
ACGTCTG5202.8430804E-86.262456410-14
GTCACTG4453.0115625E-66.045605720-24
Produced by FastQC (version 0.11.5)