FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986125
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3900.031.3607447
ACACGTC4400.029.4321428
CACGTCT4250.028.7780959
GAGCACA5850.023.3691984
AGAGCAC6200.019.7289223
GCACACG6300.019.4137956
AGCACAC6850.017.8559275
GACGCTA2600.001905901416.6044314
TTAAGTG3050.005488111614.1660912
AAGAGCA13252.2919266E-1010.32612
GAAGAGC12357.697963E-99.9569091
GTTTGAT10800.00106165797.3673531
ATCGAAA13450.00149453076.4535921
CAGTCAC4801.8537867E-76.31390520-24
AGTCACC4859.36887E-65.65368820-24
ACGTCTG5451.2221795E-55.28059810-14
CCAGTGA5304.188683E-55.16824425-29
CTCGTAT5652.17113E-55.108647335-39
Produced by FastQC (version 0.11.5)