FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1265905
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAC5901.390028E-613.4913971
CTCACAG6555.986598E-511.0477781
CTCAAAG13402.1827873E-1110.800441
CCCCGAT6355.512215E-410.25616551
CCCCAAT6900.00118114549.4386441
TTGATTC13152.2550194E-78.771573
CAGAGGG7450.00246244868.7069578
CTTTAAT14005.5302735E-78.2700511
CTCAGAA10751.1398116E-48.0777241
CCCATCT11653.295036E-58.0748351
CTCAAAC11452.3676173E-47.58388951
GTTTATT13652.9214718E-57.42184071
CCAAAAC12751.0123385E-47.37818241
CTTTCGG13051.349184E-47.2085691
CTCCTGT11400.0018760286.98238951
CTCTGCT13802.6803903E-46.81679871
CTTCTGT17351.3747307E-56.673241
CTATGAT13800.00198866336.292431
CTTTCTT36151.0913936E-116.20539761
CCCTTTT12950.0071229526.1466591
Produced by FastQC (version 0.11.5)