FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809430
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT6150.00456213639.4883031
CCTAAAT10004.464246E-58.7529591
CCCCAAC8150.0049760548.0548711
CTTACTT13201.6855116E-57.73620131
CTGGGAA13552.3868608E-57.5363731
CTTCAGT12003.710461E-47.2941331
CTACCGG12556.1988214E-46.97446971
CTTCGAA14656.689663E-56.97050241
CTCCCAT12707.098616E-46.8920941
GTTGGGA10600.00664877846.88125751
CTTACGG13802.4333957E-46.87128451
CCAGGAT11900.00271306566.74247551
CCCATGT10850.0083292236.7227031
CTTGAAA34750.06.71689941
CTCATGG11950.00283525026.71426441
CTCAAAT15501.3988999E-46.58824871
CTTGGAA22202.497909E-76.57129051
CTTTGAA31457.2759576E-116.49398141
CTCTTGT12550.00473445566.3932641
CTTACAA15157.574881E-46.25899171
Produced by FastQC (version 0.11.5)