FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691816
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAT4750.007688412410.6881631
CTTAAAG5650.002367466710.2692841
CCCAAAA7301.7520494E-49.9351811
CTCCATG7652.806776E-49.4806311
CTTTAAT10801.2149667E-69.4016251
TCAACTT7800.00362884448.3406292
CTTAAAA8900.00126822358.1490811
CTCATAA8250.00583478587.91201641
CTCAAAA9450.00228955747.6747961
CTTCATT12901.1349634E-47.30890461
CTTCAAG11100.00137906187.1873421
CTTCTCT10200.0048289487.11047271
CTTTCAT13351.7319864E-47.06253671
CTTGAAA12456.022249E-46.9905371
CTTTAAA10900.0091819916.65383671
CTTCTTT23356.8366717E-76.2121471
TTATTTA13350.0098791465.95438154
GACACAC3000.0051574155.77865545-49
CTCTTTT15250.0059030285.70702841
Produced by FastQC (version 0.11.5)