FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711724
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG1950.00649287918.7575761
CTTAGAT2901.7405291E-417.6579931
CCCCATT4006.5490804E-616.4597721
CTCCAAT6952.3719622E-914.7361661
CTTATAC4552.2190501E-514.4701311
CCTGTCT5401.1076276E-412.1924241
CTCTCAT4250.003146352512.0489851
CAACACT4550.005236904611.2545461
CCCAATG4700.00666673510.8953571
CTTATAT9451.7314778E-710.8377091
CCTTAAT4800.00779517210.6683711
CTCCGTT7602.4099856E-49.6255991
CCCAGCT6150.0044479279.5160391
CTTATGA7150.00147835679.2082641
ATACGAA9350.00200922378.90244145
CATAGAT7500.00228353038.7785451
CACCAAT7500.00228353038.7785451
CTTTACT6700.0089915458.734871
CTCCTCT6700.0089915458.734871
CTCCATT8557.8238646E-48.5560871
Produced by FastQC (version 0.11.5)