FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459513
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTT4200.003039263212.1033441
CCTTGAT6705.5301716E-611.9226971
AACAATC5150.001120894311.2316382
CTGTAAA6859.072292E-510.6014691
ATCAAAT7401.9803995E-49.8135221
CATCAAT7954.0676334E-49.1345991
CTTTTGA7650.0029162488.5435371
TTCATCC8350.0066807287.79236173
Produced by FastQC (version 0.11.5)