FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences506272
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGA2952.1701766E-417.154081
CTCCGCT3305.0985534E-415.3347071
TTCGAGT2900.003885764214.9200413
GTTAAAT3100.00595154313.9920371
CTCTTGA4750.00787063810.6535861
CTTTTCT12701.259341E-79.1076781
CATCCAT8100.00508942048.0324651
CTTCAAA10407.1265246E-47.6462891
CTTTATT9750.00320642847.41458371
CTTTTAT11600.00227041456.8552941
Produced by FastQC (version 0.11.5)