FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2312816
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACT15151.5997284E-78.2611571
CCCCATT13101.3464265E-57.8679441
CCCAAGG10607.218172E-47.63996461
CTCCAAG15602.1884935E-67.5509211
CCTCTAT15602.1884935E-67.5509211
CTTCTAT28600.07.46511461
CCCACAT15601.7699092E-57.07898861
CTAAAAG16353.4114753E-56.75426441
CTTCGAT20804.82416E-76.72503851
CTTAAAC17307.466333E-56.3833651
CTCAGAT13000.00620418046.229511
CTCACAG13050.0064565066.2056421
CTTAAAT23807.4159834E-76.18667941
CACACAT13200.0072691146.13512331
CCCACAA15650.001002276.1155221
CTCCAAT21857.671311E-66.0649271
CTCATAT24301.0760887E-66.0593811
CCCAAAT15150.0046605555.83140471
CTTCAAA45650.05.8058561
CTCATTT26801.0005751E-65.7688471
Produced by FastQC (version 0.11.5)