FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328048
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAC8758.906472E-610.0314911
CCCAAAT9301.8355266E-59.4382311
CCCATAT10659.30498E-68.9286551
CTTGAAT13904.2112333E-78.4197171
TTCACGG11302.3070352E-58.3079422
CTCCAAG8850.00110432968.2651181
CCTCAAG9953.9230046E-48.0865251
CCCAATG9050.0013774258.0824631
CTTAAAA18306.7975634E-97.99413061
CTCAAAT10758.9781336E-47.4847371
CTCAAAG14003.5422487E-57.3146291
CTTCGAT13101.2385564E-47.25879241
CTCATAT12154.1409995E-47.2243251
CAAGACA11350.0015983147.0890681
CTTCACG13802.3495225E-46.89059261
CTCCGTT11750.00230388536.8477381
CCACAAG10700.00708320556.8361021
CCTTCCT11900.0026328876.7614221
CGACAAA13450.00131831646.5260631
CTTTCAT25355.971742E-86.34800151
Produced by FastQC (version 0.11.5)