FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604901
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAATC1950.00692463618.5489922
CTCAGAT3304.970134E-415.3870961
CTCAAAT5351.09832516E-412.202851
CTTCTTA5701.981627E-411.4535521
CTCATAG5400.001621832110.7465431
CCTCTAT4800.00848268110.5471223
CAATTTA5850.00323252739.8903424
CATACAT6300.00581353629.2113231
CTTCCAG11302.1738771E-58.3452141
CTTTCAA11602.6681068E-47.5040521
CTTTTTA13702.9589166E-57.4127611
CAACAAA12851.0787281E-47.33859251
CTTTGAT10200.00481886847.11168341
TTCCATT14053.340607E-46.69347952
CTTCATT10850.0087657376.6856371
CTTCCAT11950.00299658846.67724561
CTTCTTC19007.2053517E-66.49034641
CTTTATA14050.0023617176.19551561
GTTCTTT14700.00391595955.92156461
TTGATTT14700.0040482955.90392833
Produced by FastQC (version 0.11.5)