FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683362
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCAT3100.005591643514.1253051
CTTCGAG5309.510028E-512.3929571
CAAACGA5200.001125292511.2278081
CTTCAAT11401.7753337E-910.8830941
CTTACTT4900.00924389710.4258211
CTCTTGA5950.0034517429.8125371
CTTAAAA9051.3623092E-59.6770051
CCTTGAA8054.393395E-49.0659311
TTCAATC9653.2708986E-48.2228472
CTTTTAT15602.923589E-77.953031
CCTTCAT8450.00683891967.7730971
CTTTGAT12104.04503E-47.237761
CATTTGC10000.00418790577.2152645
TCCTGAA10900.0096713676.61805442
CAAAAGA16755.3645257E-56.53558971
TTTAATT15057.9710956E-46.23108532
ATTTCCA15100.0056026625.73398456
CAAAAAG19550.00269107365.2262431
Produced by FastQC (version 0.11.5)