FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515310
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTATTA4150.003804528611.745924
CAACGAG4500.00685636710.8534191
CTTTAAA6801.2638932E-410.2605851
CTCCATT5850.0043477029.5414681
CTCAAAA7000.00187395018.9706831
CTTTAAT6350.0085323528.7901711
CATCAAT8901.9538075E-48.6235041
CTTCAAT7450.00329694788.4288291
AGTAGTG1550.00481181368.08525345-49
CTTTTTT18451.882654E-87.5633591
ATAATTT10800.00158520897.09260943
TTTTTGT12250.0059500136.25246866