FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478241
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAC3250.00788333613.4100611
CATAAGT3250.00788333613.4100611
CTTCTAT6304.9369794E-410.3768341
CTTCAAG6250.0053805089.2976441
CTTGAAA8150.00515205238.0213561
GTGTAGT1700.0078885627.651660415-19
CTTTCTT14003.8635884E-57.2637831
CTTCATC12104.2617318E-47.2037521
CAAAGAA12150.00351191486.5762651
CTTTTTT17901.4257431E-46.08696941
TGTTTTT16700.00269237325.6278264
Produced by FastQC (version 0.11.5)