FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAACGGTTCTTCCGTACTCCTCGGTAACTTATTCAAAGCAAATGACT11.1363636363636365No Hit
TTAAATTCTTGCGTATATTTACCAATAAGAGTAGTAACAGATATATAACC11.1363636363636365No Hit
TGAGCATAAAGTAAAATACTATCCTATCATGTTCAGTCGTTTAAGCGGAC11.1363636363636365No Hit
TCAACACGCTCCTGCTCAACAACGATAGTGAGCAGATACGGCTCAGGGAA11.1363636363636365No Hit
GCTCTGCCCCTGCGGCAAGCTGTCCCAGACCTGGGCGCAGGCTCACGATG11.1363636363636365No Hit
CATGATTTGTCATGTTAAAAATCGTTGTCTTATCCGCTGTTGCATGATAT11.1363636363636365No Hit
ACGGAACCCCTTGTAATCCACAACTGCCAACGTCGTGCGGGTGTGCAGCG11.1363636363636365No Hit
CGCCACAGGCCGGCAGACCCCAGCGTCTGACCGGACAGGAGATATGTTGC11.1363636363636365No Hit
CCGTTAAAAGCCGTTGTATAGCGAAACGACGCTCCAGAAAGCATCGGAAC11.1363636363636365No Hit
TAGAAATATCGGTGTACCCACTTATACTTTTGAAGGTACTCAGGTATCTA11.1363636363636365No Hit
GCCCGCGGCGCCAGCCGCGGGCCGTCGAAGCCATGTTCTTCGGGGCTTCG11.1363636363636365No Hit
ATAACGCTTATTACAAACATGGCTGCGGGGATTTTGGACAAACCGTTGTC11.1363636363636365No Hit
TACAAGCACCGATTTCTGGCGTAAGCATTTGAGTATGATGCTTTGTCTGG11.1363636363636365No Hit
AAACCTATCTGAAAAAAATTTACTTTAAGATGAAAAAATTCCTGAGATGG11.1363636363636365No Hit
AAGTGTCTCTGCTGCCTTTTTCCCCAGATTTTCCTGCTGATGAGGAAGTG11.1363636363636365No Hit
GTATTGATCAGGTCGACGTCTCCTAGAAACTCGAAAAAGGCTAGATTGCT11.1363636363636365No Hit
TGAAAAGTGTACAGAGCTTTACTGATTTTGCCTTCTTGGCTTTGAGATAT11.1363636363636365No Hit
GACTACTGGTGCCGTTACAAAGCTGGAAGGTGATGAAATCAATAAAATGA11.1363636363636365No Hit
CCGGTCCAATCCCATCCGTACGTTAGACTCCCGCAGCGCCGCCTCTGCGG11.1363636363636365No Hit
ATCATGTACACAGAGAATCTGGATGATGACAGCTTGACTCCTGGAGAGTT11.1363636363636365No Hit
CAGCAATGATGCCGGCGCGAGCGTAGTCTCTCTTTATGAAGCATTCAGGG11.1363636363636365No Hit
GTTCTTTCTTGCCGACCATGGGAAATACAGTCGAGGGGGCTTGACGGGTA11.1363636363636365No Hit
TACCATGACCATAATTAATTGTATTTAAATAGCCTTCTAATATATCCTTT11.1363636363636365No Hit
TGAACGATGATTCGATGTTTTTGAGTGACTTGAATTCCATTGTCTGATTT11.1363636363636365No Hit
AGCATCTTCTTCTAATAATCTTGATCCACTCGTTGCTACCATCACTGGAT11.1363636363636365No Hit
ATTATGAAAATATCTTAAAGAATACTAATCAAATTGATTTTGGAAAAGTT11.1363636363636365No Hit
AGGTGGGATAGGTGTGACGGGTCGCCGCGAATAATTCACCTACCAGCGCC11.1363636363636365No Hit
GTCGAACATGACCGTAGACCAGAATGTAATCAGCCAGGGCATGTTCGGCT11.1363636363636365No Hit
CTCATGGAAACGAGCCCGCTGGTGGTTTTCAAGCGTTCCGCTTCCAGGAA11.1363636363636365No Hit
TAAAGAAAAATATTATCGCAACTTACGCAATCCGTGGCTATTTCTACTGC11.1363636363636365No Hit
CAGCCGCGCACATCCGACGGGGGATTCTGGCACAAAAAGTGCTACCCCTA11.1363636363636365No Hit
CTTTAATCTGGAATTTGGAGCATGTTGTTAACATCTCCCCGCATTTAATT11.1363636363636365No Hit
AATACGAAGATCAGGTGAACCGTCTGGATACATATGCCGATCAGGTGCTG11.1363636363636365No Hit
AAATAAAAGAAATAACGGTAAGTCTTATTTTTTGCCGAATAAACGGCGTG11.1363636363636365No Hit
GGCAGCAGCGAGGGGGAGCAGCCGCGCATGGTAAGAACACCGTGGTTCGA11.1363636363636365No Hit
GCGCTCGAACTGATCTTCGCCTCGGCCATCGACCACTTCCTGATCAACAA11.1363636363636365No Hit
CTGGAAATAGCTTATCTCAAACAACCCCGGCAATTTCCGCTTCAAACTGT11.1363636363636365No Hit
CTCTTCTCATAGGCTATCTTCCACTGACTATACGCTTCATCCCAATTGTT11.1363636363636365No Hit
ATCGTTCAGATAATAGGTAATCCCGCGTTTATTCATGCCAGACAGAAACA11.1363636363636365No Hit
ATTTTAAGGTAAATAACAGCTCAATGCCGTCACTTTTCTGCGGTCAGTTC11.1363636363636365No Hit
CTTTCATATTCATGCTCCTTTCAGCATCAATCGCTTTATTGAACGGTTTT11.1363636363636365No Hit
GTCAGGCAAAGCCCGAAATTTTTATTGAACGCAAAAATGAATATAAACGA11.1363636363636365No Hit
CGGCTTGTCCGGCACGACCACGTTGCCCTTGGACTTCGACATCTTCTTGT11.1363636363636365No Hit
CGGCCCGTAGCTGGGCTTGGTGGTGTCGAAATGCCACGGATCCTGGGTCA11.1363636363636365No Hit
AAAGTCTCCCACAAAATTGGTATTTGCCATCGGATTGTCACGATAGCCAA11.1363636363636365No Hit
TCGAAAACACGTCTTGCAACATACAGCTTTCTGTCAAACTCAAGTCCTTT11.1363636363636365No Hit
GTTAAAACGATATCGCCATTTCGTTCTGCAGATATTTTTGAGATTATGCG11.1363636363636365No Hit
TCTGATGTCCAGATTCGTGAACCACGAGGCAGTGCCGATGCTTCGCCAGT11.1363636363636365No Hit
CCCGTTTATTCGGGAACAACAGCCTGCGTTGTAAGCCTAATGGTTCTTTG11.1363636363636365No Hit
GCCACATTCAAGGCCCGGCTTAACGATGCAATAACCCGAATGTCAAAATA11.1363636363636365No Hit
GCGATTCAGTTGAGCGCATTTTCACGCTATGCCCGCGTGTTGATTGGGGG11.1363636363636365No Hit
GAAAAGCGTATGTTACAAGAAGCTGTTGATGCCTTAATTGATAATGGTCG11.1363636363636365No Hit
CTGGACATCAGTTACATTATCTCCTCCGTGGGCATCAGTTCCATGTTTGT11.1363636363636365No Hit
TGTTCTCCCAATAAAGCCTGTAGCATTTCTTCCTGTCTTTCTTTATCCCA11.1363636363636365No Hit
CTGCAAGAGATTCCGAATGGCTGCTTTCCTGGACGATCAATCGCCAGCCA11.1363636363636365No Hit
CGTTTTTTTCCAAAAACTGCTGAAGGGCATAGGCTTGAAGTTTTGCTCCG11.1363636363636365No Hit
CATAACCCAGTGCTGCTGAGATAGATGTGGAACTGTGTCCTGTATCAAAA11.1363636363636365No Hit
CGCATGGCATAAAGGCGCCGTTCCGTCTCTGCATATGCCATGCGCGCGCT11.1363636363636365No Hit
ATTAATACCTTTGAATGGGTTGTGTGAAAGAGAGCTTTGTCTGTTTTAAT11.1363636363636365No Hit
GGATTCCGATACTGTTCTGGCTGCTTTTCAGGACAATCCGATCCGTGTCA11.1363636363636365No Hit
ATAGCTGCAACAGCCTCTTTTCCTCACTTATACTTTCTCTTTCCTGCGGA11.1363636363636365No Hit
GTGCCGTTCGCCATCGCGGACGCCACCACGCTGACCGAGGCCGGGTATGT11.1363636363636365No Hit
AACACCTGAATACTTATTGATAGGAGATAATAATATGACAAGTTACACGG11.1363636363636365No Hit
CAACCTCGAGGCCATGCAGCGCGCCCACGGGCCGCTGTTGGAGGTCAAGA11.1363636363636365No Hit
GGTGGACTCGATGTAGGCGGTCCACTGCGCCGCAACAGCATCGGGCGAAT11.1363636363636365No Hit
ATGCCTTCAGCGTTTATCCCTTCCCGACTTGGCTACTCGGCCATGCTCCT11.1363636363636365No Hit
CCAAGGAGAGATGTTATGCAGCAGCAGGAGGAAGCAGCACATTCGCTGCA11.1363636363636365No Hit
GGTGCGCCGTGAGCGTATAAGGTTTATCGTGTGTGACGTTTTCGACAAAG11.1363636363636365No Hit
AAGAAACAACAATAAAAGAGATTTCAGATTTTGACGGAGGGCGTAATGGG11.1363636363636365No Hit
GAGAGAAGCGACTGGCGCGCAGGATGGATACAGCAAAAAGAAAAGGCATG11.1363636363636365No Hit
ACCGCAAGCTTCATGAGCTCCGAGGGCTGGATGCGCACGCCGACGTTGAG11.1363636363636365No Hit
GGTTAAGACTATAGTAGATAAGGGAGTTCAGGTAGCTATCGTTATCGGTG11.1363636363636365No Hit
GTTAGCTTCGGGCCGCCGACAACAGGCACTTGCGCCGGGACTTCTGGACA11.1363636363636365No Hit
ATTTTTTCTTCTGTCTGTATGACCTGAAAGCCATGATCTTTTGCGTACTC11.1363636363636365No Hit
ATATATTCTGCGGAGAAGGAATCACAAAAGCATATCATTCTGAGAGTTCT11.1363636363636365No Hit
GCACACAAAATCACGTAATCGGAAAGGTTACGAAACCCACGTAATCACGT11.1363636363636365No Hit
CCATGAAACAGGAGCAGGTTACTGAACCTACTGTGGATAATATTAATGTG11.1363636363636365No Hit
GATCTTCTCTTATCTGGCCAGTTTTTTCTTCCGGCCGAAAATGAAAAAGT11.1363636363636365No Hit
CTAAAAAAGAAGTTTATATAACTGAAAGTAAGCATGTTATACAGAAATAA11.1363636363636365No Hit
GGGGAGGAAAGCATACAGTCATCACAGAGAATTTCATAAACGTATGATCC11.1363636363636365No Hit
GCCGCTGAGGGCATACAGGCGCGTTTTCGTGCGCAGAAATAGGAAAAGCC11.1363636363636365No Hit
GACCTATACAGCGGCTGCAGTGGATGAGGAGAGCGGAAAAGTTTACGGCC11.1363636363636365No Hit
CTCATAACGGAAAGGCTCAGCACAGCATATCTATTATCCAGTTTATCAAG11.1363636363636365No Hit
CCGAGACGGACACGACAATGAAAGATTGTCTGGAACGCTATCTGAGAGGT11.1363636363636365No Hit
GTCTACGATCGCATCACGATCCATGATCATAGAACCGATAACTGACTGTT11.1363636363636365No Hit
CTTCCTGCTGGCCCCCCGGCTGGGCCTCGTGGGCGTCTGGGCCGCCATGT11.1363636363636365No Hit
CAAGATTTGAAAAGGGCATTGATTTCGCATCGCAAGAGTACGGCATAAAA11.1363636363636365No Hit
CTGTTTTACAGATTCGTCACGTCGTTCAATTTTAACGGATATTAAAAATC11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGACC50.094.07
ACATGAC50.094.06
TGACCGT50.094.09
ATGACCG50.094.08
TCGAACA50.094.02
GAAAAAA50.094.094
GTCGAAC50.094.01
CGAACAT50.094.03
GAACATG50.094.04
AACATGA50.094.05
Produced by FastQC (version 0.11.5)