FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930835
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAG4300.003775230411.7610886
CTCTAAT11101.3245881E-810.5414871
CTTAGAT10054.5938978E-58.7321271
CTCACTT12901.3123044E-68.5036711
CTCTTGT22257.2759576E-128.217031
CTCACAT12305.6947654E-57.72935251
CCCCAGT11652.5628542E-47.5328651
CCCCAAT13351.5697232E-47.12142561
CTCGTAT12405.2477577E-47.0772481
CTCCTAT17852.4101773E-66.96491151
CTCATTT34150.06.8527381
CTCAGTT13150.0010246036.6736031
CTTCGTT24202.402885E-86.64832451
CTCATGT13200.00106967456.64832451
CTCCAAT24352.7162969E-86.60736941
CTTTAAT44750.06.53689961
TTCGATT27801.6607373E-96.4956742
CTCGTTT20402.7965343E-66.45278551
CGAAACT12650.00500764466.35926681
CTCAAAT26652.4198016E-86.31154251
Produced by FastQC (version 0.11.5)