FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442383
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTT2507.097333E-826.3588661
CCCTTCA2659.568101E-519.0894223
TCCCTTC2851.6696047E-417.7518222
AGCATGA4001.3372874E-414.4501529
CCTTCAG4806.237949E-412.04451754
GGTTATT4550.005197246611.2644731
CTTCAGC5300.001430592610.9082415
CAGCATG5350.001548616810.8050748
GTTTATA7550.00240234158.7281011
CCCTATT1550.00344509938.39041185-89
CTTTAAT11502.4045454E-58.2769381
TCACTAC1700.0058651367.908975140-144
CACTACT1950.0023349087.661115140-144
ACTCCAC2650.0091703595.998854105-109
Produced by FastQC (version 0.11.5)