FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027918
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT2900.00393121414.8960698
GGTAGAC3750.001339435513.4999821
CTCGTAT6354.388656E-511.3891861
CACCAAT9452.5415997E-48.4183551
CTCAAAT9753.5603365E-48.1593291
CAGGTTT8850.00129029278.1357253
AATTTCG8000.0047300248.0997375
GAGTTCA8150.0055897547.95066265
CTTCGTT10709.63501E-47.4349031
CTTTGAA17853.6858728E-77.2929071
CTTTTAT21954.2455213E-97.2486071
CATGAAT12004.0873964E-47.23213341
TTAAAGC10200.00518790877.05893753
GTATTCA11650.00248484156.79839324
CTCAGAA10650.007550896.79073521
CTCTTGA10850.00903628956.66556021
GGTTTTC12100.00370033136.54524235
TTCAATT15501.6410195E-46.507132
CAGATTT16756.48271E-56.4478663
CTTTGAG12550.0051697536.3389211
Produced by FastQC (version 0.11.5)