FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598850
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2209.313226E-1031.7369827
GAGCACA2851.6370905E-1129.4009254
CACGTCT2503.6525307E-927.9285459
AGAGCAC3051.0986696E-925.179373
ACACGTC2501.1258635E-725.135698
GCACACG2901.7733328E-824.0763326
AGCACAC3353.254172E-922.9264185
CCCCAAT2900.00475895114.4723891
AAGAGCA7201.499624E-611.6378662
GAAGAGC7452.7218306E-510.3281711
CTGCGGA7855.217719E-48.9108121
CTTTGAT8309.0863014E-48.4276961
CCCGGAA9900.0051327557.0656451
CTCGTAT2901.03858634E-46.740810435-39
TTTATTG10400.00842648656.7130323
CAGTCAC3100.00168997485.855007620-24
AGTCACC3350.00424295155.418066520-24
ACTCCAG3700.00236254495.282543715-19
Produced by FastQC (version 0.11.5)