FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605589
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5456.655173E-714.4027347
CGGACTA4150.003172521712.0372084
ACACGTC6254.2826483E-511.417448
CACGTCT6304.64522E-511.3268269
GTTTAAT8851.3687203E-48.9138971
GCACACG8950.00157615967.9730736
AGCACAC12050.00389296176.51415
CTCAAAA16403.617749E-46.1221281
AACTTAT12900.0079119436.0848765
CAACAAA24501.23086975E-55.56167651
CTTCGAT19907.1757374E-45.40575551
GAAATAG19500.0034616445.123215
Produced by FastQC (version 0.11.5)