FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907457
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG2900.003552937615.1240461
CCCAGCT7852.4493347E-611.1744561
CTTAAAC4850.00846820810.5504511
CTCTGAT7201.409057E-410.1527171
CTTCTAA6850.0010067389.6043221
CTCCATT10104.8825987E-58.6850961
CCTGTAT9452.2630792E-48.5089431
CTCATTT9953.946447E-48.0813581
CTCTGTT8800.0096935937.47609141
CCCGGAT11950.00276764556.72882941
CTTCTAT10950.0089015436.67575931
CTTTAAT13200.00107260656.64541441
CATTGAT15851.8144475E-46.45674321
CTTTAAA15308.2961714E-46.21107341
CTTTTAA14900.00418435365.88721281
CATAAAT14950.00434325875.8675231
GATTTCA17150.00375062185.4738885
CTTCAAA16100.0098522255.4484141
CTTTCAT20257.002775E-45.4147821
AAGATTT18250.00778214265.14395483
Produced by FastQC (version 0.11.5)