FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3685758
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10404.3655746E-1112.3512287
ACACGTC9301.2150849E-912.2774448
CACGTCT11854.2564352E-1010.83960159
CTTACGT9955.3331707E-58.6218731
GCACACG16057.786548E-88.0032876
CTCGTGA11100.00161107287.08456471
CTCGGTT12708.931153E-46.7549321
AGCACAC20305.4364136E-76.67926175
CTCGAAT12600.0060873356.2411641
CGACAGT14300.00340006155.99913551
CCCAAAT23506.4516043E-65.78001831
CTTTAAT53200.05.77830271
CTTCACT29352.7267015E-75.60225961
GAGCACA21757.4262454E-55.57776984
CTCAGCC19254.7968462E-45.57062531
CCCTGAA32155.1202733E-85.55907441
CTCACGG22351.0904269E-45.43769551
CCCAGCT30505.884267E-75.39102651
CTTTCAT67750.05.38151261
CTTGGAT24105.910619E-55.339481
Produced by FastQC (version 0.11.5)