FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695245
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC3056.230166E-721.051258
CACACGT2755.986929E-620.7535557
CACGTCT3158.5054126E-720.3800269
AGAGCAC4301.6494338E-514.93173
GCACACG4905.6441284E-513.103336
CTCCTAT4000.002066943812.7430521
GAGCACA5009.803289E-411.4144564
AGCACAC5702.3142039E-411.2642655
ACAACTG4500.005815015611.0973864
GTTAAAG5650.00229006510.3104331
CTCTTCT8104.3937354E-59.8887871
CTTCAAG8608.4659434E-59.3138581
CATCAAT12105.3597905E-68.42515851
ATTTGAT8659.1926206E-48.4182011
AATTTCC12006.3154584E-68.323045
CATTGAT11452.4464865E-58.2674831
ATGATTC8450.0083819787.59838063
GAAGATT10509.083201E-47.47536372
AAGCTTG9900.00424032757.20609575
CTTTCTT14656.816231E-56.95866251
Produced by FastQC (version 0.11.5)