FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1302795
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAG5451.3152542E-411.9715031
CTCACCT6100.00448560769.5074231
CTTCTAT14801.4052603E-78.3270261
CCCACAT8950.00134839278.09991
CCCATAT10558.085144E-47.55862671
CCCATTT12858.6969434E-46.7698781
CTCCGAA13100.00108238746.64068131
CTTTAAT17902.1194237E-56.479921
CTTTAAG12650.0054800296.30383541
CTTTCAC12800.00619459766.22996231
TCAATCT16904.79617E-45.9890463
CTTCCAT25706.09949E-75.9236431
CTTCAAT20601.6297499E-45.63061051
CTCCTTT22701.1138998E-45.42907431
CTTTGAT21603.2069706E-45.3699341
CATCAAG22104.4367232E-45.2484421
CCTTGAT18100.0067601735.2067591
CAATGAT19950.00377671685.08730551
CAATCTT18650.0098944085.03942354