FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763957
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAT8555.2885298E-811.8073941
CCCACAC4450.00494053411.343061
CTTTTAT14056.598566E-88.7249871
CTTCAAT11904.887861E-68.4834641
CTTTCGC8100.0052127478.012161
CTTCGTC8450.0076174667.6802961
AACATTT11050.00141760567.16891673
CTCATCC10150.00487080787.1043791
CTTTTGT12205.103602E-47.09273241
GGGGGGG2651.7339335E-47.06423825-29
CTTTAAT10450.00646200126.90042541
TCAATAT13953.2390092E-46.71107773
CTTTTGC15701.9129168E-46.4301421
CAGATAT12450.00496737756.36277343
TGATTTC14200.00288820226.08577734
CATCCAT13150.0086457326.03196861
CCCGGAA16000.00167135915.8588931
GATTTCC16950.00337849535.52219155
TTTTGCA18650.00176834515.4041857
CTTTTTT29951.9878207E-65.29685451
Produced by FastQC (version 0.11.5)