FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983935
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG4600.006370069510.9638699
CCCTCGT9251.9963238E-59.3720811
CTCAAAT16657.670731E-66.94228171
CTCAAAA25802.2628228E-96.720291
CTTAAAA25451.2036253E-86.5288481
CTTTCAT35607.2759576E-126.29083351
CTCATTC16503.56209E-46.1297191
CTTTTAG13250.00918994755.9975421
CTAAAAT19306.700336E-55.98906661
CTCAAAC13350.009931445.95261671
TCGTCGC14750.00439890665.8617038
TCGATAC16000.00169075435.85451273
AAGACTT21254.499034E-55.76444343
GTTCAAC15050.00533998435.76024871
CCAAAAT26451.1126122E-65.73574831
CTCCAAT17759.071684E-45.69804861
CTTGAAT20451.5443181E-45.6522731
CCCAGAT16650.00263409015.6406041
CACAAAT19504.9511815E-45.55716321
CTTTCGG24456.281781E-55.3185121