FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2169076
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT14156.4574124E-99.2630691
CCTCAAT17553.3942342E-98.298351
CTCTGGT12306.70812E-68.2882311
CTCAAAG24251.8189894E-128.10757351
CCCAATT10004.3486114E-48.0099821
CCTAAAT9850.00331104697.39269261
CACCAAT17401.7245766E-67.1144041
CTCTCAT13401.7321267E-47.0644351
CCCAGAT17059.699037E-66.8333621
CTTAAAG19701.6907197E-66.65342331
GTTCAAT23258.546522E-86.57711171
CTCAAAA32652.910383E-116.46775671
CCTCTAT16002.1609137E-46.3715771
CTTAAAC12800.00591849026.2577991
CACAGTT15208.047543E-46.2278571
CTATGAG17801.2852313E-46.136351
CTAAAGA13350.0091522175.99998661
CTTTATG30401.0053554E-85.9883241
CTCATTT23605.0704166E-65.8624681
CAACGGA18953.0204945E-45.76395941
Produced by FastQC (version 0.11.5)