FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005006995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005006995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073750
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTTAC3950.001991799812.80716
CCAAGGT6250.00552227639.2702471
CTAAATT6600.0086372028.7786431
CTTTGGA11750.0025565296.7801011
CTATGAT10750.0081527366.7370981
GTTTGAT12100.00347895976.5839821
CTTCAAT14504.848037E-46.49316841
CTTTGTA15952.2222602E-46.35694841
CTTTCCT14906.743069E-46.31885531
TCACTTT12800.0063958136.21058652
CTTTAAT17551.1379631E-46.19006871
TTTCCCT14700.00409272735.8992067
CTTTTGT17557.5946166E-45.77739761
CTTTGAA19103.61095E-45.6877331
CTTTTGA15550.0074474245.5889751
CTTTGAT18450.00143301995.49557351
TTGATGT15800.00908498655.4890133
CTTCCGG20850.00117245575.21034531
Produced by FastQC (version 0.11.5)