FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426236
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAC2206.364626E-419.5538949
CTCACAA7653.0065989E-49.4174681
CTCACGG10458.632677E-58.2729531
CTTTGAT27050.08.2563871
CCTAAAA8100.0052613548.0048481
CCCGAAT9150.00178335497.87362051
CTTCTAT15001.3816394E-57.20436241
CTCAAAC10100.0046897617.13303231
CTCCAAT10200.0051611677.0631011
CAACAGT10350.00594684066.9607371
CTTTAAT16354.605393E-56.60950761
CCAACGG13150.00121306446.5743231
CTCGAAG12200.00400877746.4957371
CTTGAAA22653.0208703E-66.0433951
CAACGAA13150.0087402866.0264631
GGTGGAT14450.00350048535.9828621
TCGCCCT16500.00258474545.6492983
ATTCAGA17400.0045792275.38257031
CTTTGAA23351.8694167E-45.24514681
GTTTCAT17900.0063863425.23221871