FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2167208
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAC4700.00750587410.7245019
CTATTAG7050.00138032799.2795661
CCCAAGT7700.0030760318.4962261
CCCCTAT7950.00410485178.229051
GTTAGAA10104.9311866E-47.91672561
CCCAACT10104.9311866E-47.91672561
CCCAAGA12054.753234E-57.84206721
CTCAAAG22257.8216544E-117.8407121
CTCACGA9400.0021303787.73297171
CCCGTCT11301.931661E-47.71928551
CTCCGAT16356.6246866E-77.5579751
CCCAGCT18306.4284905E-87.5470421
CTCACTT10658.694077E-47.507881
CTACAAT20407.221388E-97.48278671
CCTAAAT10800.00100905747.4036041
CCCATTT21301.659646E-87.1666131
CTCAAAT19401.7903949E-77.11911731
CTCCAGG12305.1248714E-47.0917011
CTCAAAA28759.094947E-127.0793681
CTCAGGA15501.9331575E-57.03450971
Produced by FastQC (version 0.11.5)