FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51479182
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCC301950.042.6977541
TTAGTCC241900.042.325471
TAAGTCG53900.041.238811
TATGTCG92150.040.5436171
ATACTCG78250.040.4076581
AATGTCG95800.038.9456181
TTAGTCG74650.038.9377331
AATGTCC383150.036.033391
GTTGTCC338200.036.023471
TAAGTTG115400.034.9846421
ATACTCC203350.034.825351
GTAGTCG60750.034.404431
TTTGTCC287200.033.3658521
ATACTTG132650.033.0131381
TTAGTCA172250.032.964581
TAAGTCC156500.032.906641
TATGTCA191000.032.1869771
TATGTGG160750.032.068391
TTAGTGC113350.031.925041
GTAGTCA121450.031.266711
Produced by FastQC (version 0.11.5)