FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51479182
Sequences flagged as poor quality0
Sequence length57
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1027030.19950394705183935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGTCC284900.042.679981
TATGTCG94500.042.5759661
TTAGTCC270000.039.898111
TAAGTCG65450.039.8914341
GTTGTCC394450.037.704171
AATGTCC321950.036.683391
AATGTCG105450.036.0028951
TTAGTCG101150.035.365611
TAAGTTG171300.035.246311
ATACTCC149500.035.030681
ATACTCG68350.034.8415681
TAAGTCC143600.034.4280741
TTTGTCC286500.034.40541
TTAGTCA218850.033.8795171
TATGTCA216900.033.1966671
ATACTTG142800.032.2819371
TTAGTTG212100.032.1928371
TTAGTGC136050.031.953261
GTTGTCG131800.031.8229061
TATGTTC144150.031.5020371
Produced by FastQC (version 0.11.5)