FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468727
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATT10458.295516E-69.0109621
CCCAAAT16701.4770194E-98.6747641
CTTTCGA17955.7079887E-98.0706711
CTTACGA14205.914493E-67.65150741
GTTTGAA30252.3101165E-106.46520761
CCCCAAT12900.00677954736.17656561
GGGGGGG2650.0090827666.004943105-109
CTCTTAC13350.0096666325.9683671
CTCCGAA22151.243913E-55.8863071
CTCAAAA31502.4627298E-85.74875161
ATTTAGC15100.00557116935.7384086
CTTTGAA43709.094947E-125.6356181
CTTTAAG20851.9636011E-45.55850551
CTTACAT18450.00143313775.4963671
CTTCGAA15950.0098474825.44961
CTTCGAT26958.945255E-65.37545631
CTGAACT17950.0061991645.24593641
CTCCATT29005.751768E-65.245241
CTTATCT32151.2891214E-65.1819231
CTAAAAT30853.228377E-65.1654911
Produced by FastQC (version 0.11.5)