FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678586
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2850.034.2394527
ACACGTC3400.028.7007188
CACGTCT4050.025.8154629
GCACACG5100.023.2322036
AGAGCAC5101.8189894E-1220.4974943
AGCACAC4852.7284841E-1120.118615
GAGCACA5955.2204996E-1016.3992024
GAAGAGC9001.3342287E-811.64355851
AAGAGCA8558.405368E-811.4190432
AAATTCG5600.00305933049.9566586
AGTCACT2653.2426233E-57.36471320-24
TCGCCGA10800.00156285567.10291622
CAGTCAC3402.3824432E-67.061981145
CGGAAAT12350.0062619036.2224691
ACTCCAG3604.102647E-56.1949815-19
ACGTCTG4352.6691796E-66.088439510-14
CCAGTCA4854.3113687E-76.03602820-24
CGTCTGA4504.7500052E-65.885491410-14
ACTGACT3653.2880335E-45.72831925-29
CTCCAGT4402.0744945E-55.70219815-19
Produced by FastQC (version 0.11.5)