FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417229
Sequences flagged as poor quality0
Sequence length100-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGAC5451.4004575E-414.375413145
CTCCGAT10457.5259374E-47.60966871
CAAGTAT10350.0057516856.98471981
AATTGAA10650.0078645546.7625815
TTCAATC15551.6883913E-46.49341342
CTTCAAT15359.878626E-46.1224371
CTCCGCT14400.00324071086.0243211
CTTTCGC14700.0040785325.9013761
CTTCCGG27804.5534034E-75.72093771
CCAGAAT21956.9964786E-55.5989131
CAAAAAA22706.736809E-45.0954611