FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570521
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2155.7843863E-1033.1810079
CACACGT2152.114939E-829.8629067
ACACGTC2903.8329927E-722.139748
AGCACAC3401.7666807E-618.882245
GCACACG3903.2301978E-718.2920916
AGAGCAC3805.0730687E-616.906493
GAGCACA3855.745007E-616.6869244
CTTCGTT4907.5880095E-411.767891
TGATACG4250.00379557211.7499796
GAAGAGC7151.511566E-410.0808851
CTTGAAT5950.00382408859.6912041
TCACTCG2000.0046716159.350971145
AAGAGCA7904.5392034E-49.0358182
AGTCACT2101.0608794E-58.8375820-24
CACTCGG2404.7706673E-47.136418325-29
CAGTCAC2803.781433E-46.62818520-24
ACTCCAG3152.3022662E-46.34070315-19
CGTCTGA3200.0019067195.796103510-14
Produced by FastQC (version 0.11.5)