FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084044
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC22350.018.4334036
CCGATCT24000.015.3556627
CTTCCGA34950.012.8225344
TTCCGAT44950.010.1305535
CGATCTA5900.00349966839.7982298
GACGCTC22301.8189894E-128.4300791
CGATCTC23655.456968E-127.94423068
GCTCTTC54750.07.92374131
ACGCTCT28200.07.4332422
TCTTCCG58900.07.2404593
CTCTTCC65450.06.9576132
CGCTCTT34750.06.86417683
CTCCCTT21651.8614628E-76.6793661
CTCGAAT13100.00111650426.623281
CTCAAAT23558.489933E-76.1404791
CTAATGT13000.0074842276.1180431
CACGAAT18702.7782636E-45.79979751
CTTAAAT24401.0039237E-55.63024041
GTCCAGT15450.00707509375.61585571
CTCAGCT18300.00132269595.53146461
Produced by FastQC (version 0.11.5)