FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828390
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGA4907.7621127E-411.737321
CGTAAGA5700.002741080710.0899761
CTCCAAT6800.00109641289.5149971
GGTGTAG6400.0071274898.9863851
CTATGAT9854.234151E-48.0284461
ATCAAGT10004.9801404E-47.9080191
CCTGCTT12407.765059E-57.5369681
CTGCCGA10300.0060710076.945178
CTGCAAT13350.00147159296.46211961
CTTTTGA12250.0042722236.45552541
CTTATAT12950.00761489946.10657841
CATCAAT17206.446117E-45.8515991
AGATTTC14750.0047546125.81981664
AAGATTT16050.00190784115.7944823
CTTTCAT15300.00675097675.63851641
CTTCAAC15350.00699868335.62014961
CAAAGAT19605.648759E-45.50186871
CTTTTCT21302.9539657E-45.4002691
CTTCCAT17700.00573018575.2801361
GATTTCT17850.00670438735.20985035