FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407363
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT1257.3341653E-944.6136979
CACACGT1551.9865838E-631.4814387
ACACGTC2301.5490514E-624.2465748
AGAGCAC2402.2430177E-623.2363013
AGCACAC2508.064669E-519.5184925
GCACACG3202.6955046E-517.4272256
GAGCACA3003.2515547E-416.2674084
GAAGAGC5150.001489495910.8552221
CGAAAAT5300.001892062410.5479991
AACCTGC4700.00952474710.3821765
AAGAGCA5600.00304419529.960862
Produced by FastQC (version 0.11.5)