FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840708
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2601.364242E-1030.137339
ACACGTC2706.710252E-926.3828498
CACACGT3453.6325218E-922.712197
ATTTAGG2800.003340878315.2643626
GCACACG5507.513918E-714.24673756
GAGCACA5507.513918E-714.24673754
AGCACAC5952.633857E-511.9720495
CGGCTTA4200.003515162311.8722815
AGAGCAC6002.869409E-511.8722813
CCCCGCT4300.004107949311.6273021
GTCACAA4700.0079654210.6377451
AAGAGCA12156.749389E-57.6226182
GAAGAGC9900.0041938677.2146321
TTTCCCT11600.0026568636.75491868
TCAGGAT12550.0060494586.24359043
AGTCGAG3457.5410964E-45.781766430-34
AGTCACA3855.0280883E-45.551271420-24
Produced by FastQC (version 0.11.5)