FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336977
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAG2950.003856468514.9395691
GAGTGGT4856.9877156E-411.8862089
CTCAAAT10901.12552036E-48.08655551
CTTGCAT10901.12552036E-48.08655551
CAATCGT10154.6448424E-47.9604111
CCCCTTC8700.0083899717.5985741
CTTAAAT12607.265031E-57.57847171
CCCAAAT10050.00369936277.30874441
CCCAGCT15551.7447454E-57.0854871
CTATAAT15852.280121E-56.95137641
CTTCAAC15902.3827808E-56.92951771
CTTGAAT11900.00251958646.78976151
CAAAGAA32503.6379788E-126.7802661
CCCAGAT12200.00327249386.62280131
CTCAAAG13600.00142538846.4811371
CTCCAGA13650.0014857136.4573961
CTTGTAT13050.00660691646.19143061
CCTTTAT19104.5184082E-56.1531211
CTTATTT20551.3032214E-45.71895931
CTTGTTT19554.0913877E-45.6357711
Produced by FastQC (version 0.11.5)