FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094017
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACT6905.75199E-712.6008551
CTCAATT6708.917231E-49.7327491
CTCAGCT8306.0730625E-59.6024581
CTCAAAT9904.152271E-69.514281
CTTCAAG23553.6379788E-127.9992681
ACGGTTG8250.0061021457.8734435
CCCAAAG8450.0073035987.71709061
GTTTAAT9700.0029895827.46957871
CTTTAAT13652.8712873E-57.43127251
CTATGAT11753.1423251E-47.3996511
CTCCATT11853.4640296E-47.33720641
AAATACG12205.0581375E-47.0990066
CTTTTAA16355.5931832E-67.0903891
GTTTCAC10300.00557341757.007117
CTTTGTT16556.7018173E-67.00470451
CTTTGCT14808.335717E-56.85384271
CTTCGAT11700.00243343416.8121
CTTCGCT10950.0096986256.6168871
CTTTTGT15651.7274525E-46.48158931
CTTCTCA16052.3963719E-46.3200541
Produced by FastQC (version 0.11.5)