FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695261
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT7151.3264555E-410.2147831
CTCACGA6607.2235725E-49.9594131
CTCTCGG9101.5197525E-48.8284911
CACACAT8050.00440092768.1654811
CTATTCT12751.0426254E-58.0196061
CTAAAAG10104.6847778E-47.9543821
CTTACGG10150.00430835557.19563441
CTCGCTC11500.00186666216.9860231
CCTCGAT14706.8769754E-56.955781
CCTCCAT18654.8796355E-66.6574091
CTCACGG11050.0098090136.6095651
CTCCAAC14554.5689492E-46.52552561
CTCTGAT12550.0046706186.40153461
GTTCGGA13850.00186747396.3280021
CCCAGAT14000.00210802636.2602021
CTATAAT17751.18623626E-46.17203041
CCCCAAA14250.0025712656.1503741
CATAGAT14300.0026741816.12886951
CTTTCGA13150.0075890566.10944941
CTTCGAT17054.7175487E-45.99706551
Produced by FastQC (version 0.11.5)