FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005007107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005007107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665399
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2406.002665E-1132.3160137
ACACGTC2551.2187229E-1030.4150718
CACGTCT2452.6520866E-928.7786399
GAGCACA4009.731593E-1021.155484
GCACACG4554.833055E-918.5982256
CGAACCC2550.007373440518.347431145
AGCACAC4004.759877E-717.6295665
AGAGCAC4451.4508205E-615.8468013
GAAGAGC8308.2636287E-48.5089671
AAGAGCA9804.891765E-47.92067342
GGGGTAC2050.00667541156.878796625-29
GCCAAGG11800.00347603116.58363251
ACCCAAT2650.00168768896.385599625-29
CGTCTGA4602.0628815E-45.211436310-14
GTCACCC3850.0034268315.1278320-24
Produced by FastQC (version 0.11.5)